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Volume 45 - Number 2 - 2010
Article

 


Acetylcholinesterase activity, antioxidant defenses, and lipid peroxidation in the clam Semele solida: Can this species be used as a bioindicator?

Benjamin Srain1 and Anny Rudolph2

 

1Laboratorio de Geoquímica Orgánica Marina, Departamento de Oceanografía, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Casilla 160-C, Concepción, Chile

2Departamento de Química Ambiental, Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Alonso de Ribera 2850, P.O. Box 297, Concepción, Chile

 

E-mail: annyr@ucsc.cl

 

We analyzed the relationships between biochemical parameters: acetylcholinesterase activity (AChE); glutathione S-transferase activity (GST); glutathione concentration (GSH) and lipid peroxidation; in the bivalve mollusc Semele solida, with the effects of environmental stress (anthropogenic activity), from three estuaries in Eastern South Pacific bays: Coliumo estuary (in Coliumo Bay), Lenga estuary (in San Vicente Bay) and Andalién estuary (in Concepción Bay). Coliumo is the estuary with minor environmental stress. Thirty juveniles from each site were selected for individual analyses in homogenized of gill or digestive gland tissue to assess AChE activity, GST activity, GSH concentration, malonyldialdehyde concentration (MDA) (lipid peroxidation), and protein content. The biochemical parameters analyzed in specimens from Coliumo estuary, differed significantly from that in Andalién estuary, with lower AChE activity in gill tissue (2189.9 ± 189.6 µmol minˉ¹mg proteinˉ¹) and intracellular GSH levels (59.8 ± 13.3 µM), and the highest lipid peroxidation (31.9 ± 7.4 nmol MDA mLˉ¹) and GST activity (614.9 ± 92.3 µmol minˉ¹mg proteinˉ¹). The biochemical parameters in S. solida were closely related to the sector’s stress. Due to this bivalve sensitivity, is recommended as bioindicator for use in programs of environmental alertness in the Eastern South Pacific coastal zone

 

Key words: Biomarkers, bioindicadores, marine bivalves, programs of environmental alertness

 

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