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Volume 44 - Number 2 - 2009

Enterovirus in Valparaiso natural water resources: a methodological proposal for its analysis

Maritza Soler1, Soledad Lobos2, Marcos Lorca3 y Ema Navarrete4


1Depto. de Química y Bioquímica, Facultad de Ciencias, Universidad de Valparaíso, Gran Bretaña 1111 Playa Ancha, Valparaíso, Chile
2Depto. de Ciencias Químicas y Recursos Naturales, Facultad de Farmacia, Universidad de Valparaíso, Gran Bretaña 1093 Playa Ancha, Valparaíso, Chile
3Tesista Proyecto DIPUV 04/2001, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Leopoldo Carvallo 270, Playa Ancha, Valparaíso, Chile
4Depto. de Pre-Clínica, Facultad de Medicina, Universidad de Valparaíso, Hontaneda 2653, Valparaíso, Chile




In Chile, water sanitary quality is determined by the concentration of fecal indicator bacteria. However, Numberus investigations have shown that achieving minimum fecal coliform standards do not predict viral contamination, such as enteric viruses. Enteroviruses are microorganisms causing several infections and massive outbreaks of illness which represent a serious risk for public health. In the present study, performed during 2001-2003 in Valparaiso, central Chile, natural water samples were collected from five streams and processed for enterovirus detection by tissue culture and reverse transcription (RT) associated to polymerase chain reaction (PCR). None enterovirus was found in any of the natural stream waters. Simulation of an infection, in drinking water and water from one stream was used to check RT-PCR detection of enterovirus


Key words: Aquatic pollution, cell culture, RT-PCR, public health, virus infections




1Laboratorio de Zooplancton, Instituto de Ciencias del Mar y Limnología,Universidad Nacional Autónoma de México. A. P. 70-305, 04510 México, D. F. México
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