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Volume 44 - Number 3 - 2009

Application of calcein for estimating growth of the yellow clam Mesodesma mactroides Reeve, 1854

Mauro L. Lepore1, Pablo E. Penchaszadeh1, José E. F. Alfaya1 y Marko Herrmann2


1Lab. Invertebrados, Depto. de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 4° piso, Lab. 19, C1428EHA, Buenos Aires, Argentina
2Alfred Wegener Institute for Polar and Marine Research (AWI), Section of Marine Animal Ecology, Postfach 120161, D-27515, Bremerhaven, Germany




Growth rates of Mesodesma mactroides were estimated comparing two different methods at the intertidal of the exposed sandy beach Santa Teresita, Argentina. Results
of a short tagging-recapture experiment using the in situ fluorescent marking (MFI) method and subsequent size-increment analysis were compared with results from length-frequency distributions (DFT) analysis from a 25 month quantitative sampling. Residuals, derived from MFI method and DFT analysis, were of similar magnitude and distribution, suggesting that both methods are equally appropriate to estimate growth of M. mactroides. Calcein was useful as non-lethal growth marker for M. mactroides, emitting a bright green fluorescence band under blue light


Key words: In situ fluorescent marking, comparison of methods, daily growth rate, length-frequency distribution, overall growth performance index




1Laboratorio de Zooplancton, Instituto de Ciencias del Mar y Limnología,Universidad Nacional Autónoma de México. A. P. 70-305, 04510 México, D. F. México
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